Anticorps Recombinant de lapin anti-TGFBR2

TGFBR2 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250238A7

N° de cat : 98438-1-PBS

Synonymes

EC:2.7.11.30, TbetaR-II, TGF beta receptor type 2, TGF-beta receptor type II, TGF-beta receptor type-2

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Galerie de données de validation

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  FC experiment of human PBMCs using 98438-1-RR (same clone as 98438-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TGFBR2 Rabbit RecAb (98438-1-RR, Clone: 250238A7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98438-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98438-1-RR (same clone as 98438-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TGFBR2 Rabbit RecAb (98438-1-RR, Clone: 250238A7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98438-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98438-1-RR (same clone as 98438-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TGFBR2 Rabbit RecAb (98438-1-RR, Clone: 250238A7) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98438-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98438-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 98438-1-PBS against Human TGFBR2 was performed. The affinity constant is 0.937 nM.

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Informations sur le produit

98438-1-PBS cible TGFBR2 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TGFBR2 Protéine recombinante Eg2943
• Nom complet: transforming growth factor, beta receptor II (70/80kDa)
• Masse moléculaire calculée: 65 kDa
• Numéro d’acquisition GenBank: NM_003242.5
• Symbole du gène: TGFBR2
• Identification du gène (NCBI): 7048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 is a serine/threonine kinase and the primary ligand-binding receptor. After binding of the ligand TGF-β1, TGFBR2 forms a heterodimer complex with TGFBR1, causing conformational changes and signal propagation via canonical (SMAD-dependent) and non-canonical (SMAD-independent) routes. Upon translocation into the nucleus, activated SMAD complexes can interact with various transcription factors to control target gene expression (PMID: 31454892).

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