Anticorps Monoclonal anti-TGN46

TGN46 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F6D5

N° de cat : 66477-1-PBS

Synonymes

TGOLN2, 1F6D5, hTGN46, hTGN48, hTGN51

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Galerie de données de validation

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  WB analysis of HeLa using 66477-1-Ig (same clone as 66477-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  WB analysis using 66477-1-Ig (same clone as 66477-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66477-1-PBS cible TGN46 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TGN46 Protéine recombinante Ag4470
• Nom complet: trans-golgi network protein 2
• Masse moléculaire calculée: 447 aa, 47 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC028219
• Symbole du gène: TGN46
• Identification du gène (NCBI): 10618
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

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