Anticorps Monoclonal anti-TGN46

TGN46 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1F6D5

Avis (1)

N° de cat : 66477-1-Ig

Synonymes

TGOLN2, 1F6D5, hTGN46, hTGN48, hTGN51

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Galerie de données de validation

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  WB analysis of HeLa using 66477-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66477-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules L02, cellules LNCaP, cellules SMMC-7721, tissu hépatique de porc
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66477-1-Ig cible TGN46 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TGN46 Protéine recombinante Ag4470
• Nom complet: trans-golgi network protein 2
• Masse moléculaire calculée: 447 aa, 47 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC028219
• Symbole du gène: TGN46
• Identification du gène (NCBI): 10618
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Publications

Species	Application	Title
		Sci Adv; Isocyanic acid-mediated NLRP3 carbamoylation reduces NLRP3-NEK7 interaction and limits inflammasome activation; Authors - Zhenxing Zhang
human		Arterioscler Thromb Vasc Biol; IL-1β Induces LDL Transcytosis by a Novel Pathway Involving LDLR and Rab27a; Authors - Erika Jang
pig		J Virol; Porcine deltacoronavirus accessory protein NS6 harnesses VPS35-mediated retrograde trafficking to facilitate efficient viral infection; Authors - Puxian Fang
mouse		J Cell Physiol; Locational and functional characterization of PI4KB in the mouse embryo; Authors - Haoyu Zhao
mouse		Int J Biol Sci; Annexin A5 Derived from Cell-free Fat Extract Attenuates Osteoarthritis via Macrophage Regulation; Authors - Zhuoxuan Jia
human	WB	Mol Metab; Tetraspanin7 in Adipose Tissue Remodeling and Its Impact on Metabolic Health; Authors - Shino Nemoto

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Karla (Verified Customer) (12-20-2022)

Proteintech has the best for IMF. Made my images clear and was picked up easily on the microscope.

• Applications: Immunofluorescence

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