Anticorps Recombinant de lapin anti-TGN46

TGN46 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230376D6

N° de cat : 83099-2-PBS

Synonymes

TGOLN2,TGN46, TGOLN2, TGN38 homolog, TGN38, hTGN51

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Galerie de données de validation

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  WB analysis using 83099-2-RR (same clone as 83099-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83099-2-RR (TGN46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 83099-2-RR (same clone as 83099-2-PBS)

  IP result of anti-TGN46 (IP:83099-2-RR, 4ug; Detection:83099-2-RR 1:2000) with HeLa cells lysate 980 ug. This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83099-2-RR (same clone as 83099-2-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 83099-2-RR (TGN46 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83099-2-RR (same clone as 83099-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGN46 antibody (83099-2-RR, Clone: 230376D6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83099-2-RR (same clone as 83099-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGOLN2,TGN46 antibody (83099-2-RR, Clone: 230376D6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83099-2-RR (same clone as 83099-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TGOLN2,TGN46 Recombinant antibody (83099-2-RR, Clone:230376D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83099-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83099-2-RR against Human TGOLN2,TGN46 were performed. The affinity constant is 0.633 nM.

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Informations sur le produit

83099-2-PBS cible TGN46 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TGN46 Protéine recombinante Ag4470
• Nom complet: trans-golgi network protein 2
• Masse moléculaire calculée: 447 aa, 47 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC028219
• Symbole du gène: TGN46
• Identification du gène (NCBI): 10618
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

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