Anticorps Monoclonal anti-TH

• Phare
• Validé par KD/KO

TH Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

21

CloneNo.

2H7B7

N° de cat : 66334-1-Ig

Synonymes

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 66334-1-Ig

  WB result of TH antibody (66334-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TH transfected SH-SY5Y cells.

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  WB analysis of SH-SY5Y using 66334-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 66334-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 66334-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 66334-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66334-1-Ig(TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules SH-SY5Y
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:20000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 7 publications below

Informations sur le produit

66334-1-Ig cible TH dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TH Protéine recombinante Ag23075
• Nom complet: tyrosine hydroxylase
• Masse moléculaire calculée: 528 aa, 59 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC104967
• Symbole du gène: TH
• Identification du gène (NCBI): 7054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.

Publications

Species	Application	Title
human	WB	Mol Oncol; Norepinephrine-CREB1-miR-373 axis promotes progression of colon cancer.; Authors - Jia Han
	WB	Front Cell Dev Biol; RAB39B Deficiency Impairs Learning and Memory Partially Through Compromising Autophagy.; Authors - Mengxi Niu
human	IF	Antioxidants (Basel); Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency; Authors - Xiao Sun
	WB,IF	Bone Joint Res; High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice; Authors - Hecheng Zhou
	IHC	Biomed Pharmacother; Nardosinone regulates the slc38a2 gene to alleviate Parkinson's symptoms in rats through the GABAergic synaptic and cAMP pathways.; Authors - Li-Hua Bian
	IHC	J Neuropathol Exp Neurol; Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.; Authors - Wenfeng Liu