Anticorps Monoclonal anti-TH

• Phare
• Validé par KD/KO

TH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H7B7

N° de cat : 66334-1-PBS

Synonymes

tyrosine hydroxylase, 2H7B7, DYT14, DYT5b, EC:1.14.16.2

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

  WB result of TH antibody (66334-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TH transfected SH-SY5Y cells. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66334-1-Ig(TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66334-1-PBS cible TH dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TH Protéine recombinante Ag23075
• Nom complet: tyrosine hydroxylase
• Masse moléculaire calculée: 528 aa, 59 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC104967
• Symbole du gène: TH
• Identification du gène (NCBI): 7054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.

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