Anticorps Monoclonal anti-TH1L

TH1L Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A10G2

N° de cat : 67380-1-PBS

Synonymes

HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L

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Galerie de données de validation

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  WB analysis using 67380-1-Ig (same clone as 67380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67380-1-Ig (same clone as 67380-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67380-1-Ig (same clone as 67380-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67380-1-Ig (same clone as 67380-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67380-1-Ig (same clone as 67380-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67380-1-Ig (same clone as 67380-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67380-1-Ig (same clone as 67380-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67380-1-Ig (same clone as 67380-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67380-1-Ig (TH1L antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67380-1-Ig (same clone as 67380-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TH1L antibody (67380-1-Ig, Clone: 1A10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67380-1-Ig (same clone as 67380-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TH1L antibody (67380-1-Ig, Clone: 1A10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67380-1-Ig (same clone as 67380-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TH1L (67380-1-Ig, Clone:1A10G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67380-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67380-1-PBS cible TH1L dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TH1L Protéine recombinante Ag24894
• Nom complet: TH1-like (Drosophila)
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 60-66 kDa
• Numéro d’acquisition GenBank: BC014952
• Symbole du gène: TH1L
• Identification du gène (NCBI): 51497
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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