Anticorps Polyclonal de lapin anti-THAP1

• Phare
• Validé par KD/KO

THAP1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12584-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of A549 using 12584-1-AP (same clone as 12584-1-PBS)

  WB result of THAP1 antibody (12584-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THAP1 transfected A549 cells. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12584-1-AP (same clone as 12584-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 12584-1-AP (same clone as 12584-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12584-1-AP (same clone as 12584-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12584-1-AP (same clone as 12584-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 12584-1-AP (same clone as 12584-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12584-1-AP (same clone as 12584-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 12584-1-AP (same clone as 12584-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12584-1-AP (same clone as 12584-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12584-1-AP (same clone as 12584-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 12584-1-AP (same clone as 12584-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SGC-7901 using 12584-1-AP (same clone as 12584-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12584-1-AP (same clone as 12584-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12584-1-AP (same clone as 12584-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12584-1-AP (same clone as 12584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12584-1-AP (THAP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12584-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12584-1-PBS cible THAP1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: THAP1 Protéine recombinante Ag3285
• Nom complet: THAP domain containing, apoptosis associated protein 1
• Masse moléculaire calculée: 213 aa, 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC021721
• Symbole du gène: THAP1
• Identification du gène (NCBI): 55145
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

THAP1 belongs to the THAP1 family. It is a DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. THAP1 may also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Mutations in THAP1 have been associated with dystonia 6. THAP1 encodes a transcription factor with mostly unknown targets. It regulates the expression of DYT1 (TOR1A), another dystonia-causing gene. After characterization of the TOR1A promoter, THAP1 binds to the core promoter of TOR1A. Wild type THAP1 represses the expression of TOR1A, whereas dystonia 6-associated mutant THAP1 results in decreased repression of TOR1A. Catalog#12584-1-AP is a rabbit polyclonal antibody raised against full-length of huamn THAP1.

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