Anticorps Polyclonal de lapin anti-THAP2

THAP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 20853-1-AP

Synonymes

THAP domain-containing protein 2

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Galerie de données de validation

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  WB analysis of HEK-293 using 20853-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20853-1-AP (THAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20853-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20853-1-AP (THAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 20853-1-AP

  Immunofluorescent analysis of Hela cells, using THAP2 antibody 20853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 20853-1-AP

  Immunofluorescent analysis of HepG2 cells, using THAP2 antibody 20853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

20853-1-AP cible THAP2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: THAP2 Protéine recombinante Ag14775
• Nom complet: THAP domain containing, apoptosis associated protein 2
• Masse moléculaire calculée: 228 aa, 26 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC008358
• Symbole du gène: THAP2
• Identification du gène (NCBI): 83591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

THAP2, also named as THAP domain-containing protein 2, is a 228 amino acid protein, which contains one THAP-type zinc finger. Members of the THAP (thanatos-associated protein) family of proteins contain a well conserved DNA-binding domain known as the THAP-type zinc finger motif. Proteins containing the THAP-type zinc finger motif are commonly involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification

Publications

Species	Application	Title
rat	WB	Cancer Cell Int; The involvement of miR-100 in bladder urothelial carcinogenesis changing the expression levels of mRNA and proteins of genes related to cell proliferation, survival, apoptosis and chromosomal stability.; Authors - Denis R Morais
human	WB	Clinics (Sao Paulo); MicroRNA 100: a context dependent miRNA in prostate cancer.; Authors - Leite Katia R M KR
Human	WB	Nat Commun; Reactivity-dependent profiling of RNA 5-methylcytidine dioxygenases; Authors - A. Emilia Arguello

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