Anticorps Polyclonal de lapin anti-Thrombomodulin

Thrombomodulin Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 14318-1-AP

Synonymes

CD141, Fetomodulin, THBD, THRM, thrombomodulin, TM

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Galerie de données de validation

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  WB analysis of human heart using 14318-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14318-1-AP (Thrombomodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14318-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14318-1-AP (Thrombomodulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14318-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14318-1-AP (Thrombomodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14318-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14318-1-AP (Thrombomodulin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14318-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14318-1-AP (Thrombomodulin antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of A431 using 14318-1-AP

  1X10^6 A431 cells were stained with .2ug Thrombomodulin antibody (14318-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules THP-1, tissu cardiaque humain
• Résultats positifs en cytométrie: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

14318-1-AP cible Thrombomodulin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Thrombomodulin Protéine recombinante Ag5558
• Nom complet: thrombomodulin
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC035602
• Symbole du gène: THBD
• Identification du gène (NCBI): 7056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

Publications

Species	Application	Title
rat	IF	Bioengineered; The crescendo pulse frequency of shear stress stimulates the endothelialization of bone marrow mesenchymal stem cells on the luminal surface of decellularized scaffold in the bioreactor; Authors - Yuhao Jiao
human	WB	Front Cell Dev Biol; Crocin Improves Endothelial Mitochondrial Dysfunction via GPx1/ROS/KCa3.1 Signal Axis in Diabetes.; Authors - Xuemei Li
human	IHC	Diagnostics (Basel); Urinary SARS-CoV-2 RNA Is an Indicator for the Progression and Prognosis of COVID-19.; Authors - Lu Zhang
human	IHC	Interact Cardiovasc Thorac Surg; Atrial endocardial expression of von Willebrand factor and thrombomodulin is associated with recurrence after minimally invasive surgical atrial fibrillation ablation.; Authors - Kang An
human	WB,IF	Ther Apher Dial; Decreased Expression of Thrombomodulin in Endothelial Cells by Fibroblast Growth Factor-23/α-Klotho.; Authors - Kenji Tanaka
human	IHC	Clin Appl Thromb Hemost; Endocardial Changes in Nonvalvular Atrial Fibrillation Without Atrial Thrombus-Thrombomodulin and Tissue Factor Pathway Inhibitor.; Authors - Kang An

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