Anticorps Monoclonal anti-CD141/Thrombomodulin

CD141/Thrombomodulin Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

5A4C5

N° de cat : 67831-1-Ig

Synonymes

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

---

Galerie de données de validation

• 

  WB analysis using 67831-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of human placenta using 67831-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67831-1-Ig (THBD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 67831-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67831-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67831-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67831-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67831-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HUVEC, cellules THP-1, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu d'amygdalite humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67831-1-Ig cible CD141/Thrombomodulin dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD141/Thrombomodulin Protéine recombinante Ag5723
• Nom complet: thrombomodulin
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC035602
• Symbole du gène: THBD
• Identification du gène (NCBI): 7056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thrombomodulin, also known as THBD and CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

{{ptg:RelatedPrimaryAntibodies}}