Anticorps Polyclonal de lapin anti-Thrombospondin 3-Specific

• Phare
• Validé par KD/KO

Thrombospondin 3-Specific Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 19727-1-AP

Synonymes

THBS3, thrombospondin 3, Thrombospondin 3-Specific, TSP3

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Galerie de données de validation

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  IHC staining of human liver using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu hépatique humain, tissu de côlon humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

19727-1-AP cible Thrombospondin 3-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: thrombospondin 3
• Masse moléculaire calculée: 104 kDa
• Numéro d’acquisition GenBank: NM_007112
• Symbole du gène: Thrombospondin 3
• Identification du gène (NCBI): 7059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

THBS3, also named as TSP3, belongs to the thrombospondin family. THBS3 is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. THBS3 can bind to fibrinogen, fibronectin, laminin and type V collagen. The antibody is specific to THBS3.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.; Authors - Davy Vanhoutte
human	IF	Front Cell Dev Biol; Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.; Authors - Mansheng Li
mouse	WB	Mol Cell Biochem; Microrna363-5p targets thrombospondin3 to regulate pathological cardiac remodeling; Authors - Yu-Kun Ma
mouse	WB,IF	Nat Commun; Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability.; Authors - Tobias G Schips ; KO Validated

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