Anticorps Polyclonal de lapin anti-THEM2

THEM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16782-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 16782-1-AP (same clone as 16782-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 16782-1-AP (same clone as 16782-1-PBS)

  IP result of anti-THEM2 (IP:16782-1-AP, 4ug; Detection:16782-1-AP 1:2000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16782-1-AP (same clone as 16782-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16782-1-AP (same clone as 16782-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16782-1-AP (same clone as 16782-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using THEM2 antibody (16782-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

16782-1-PBS cible THEM2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: THEM2 Protéine recombinante Ag10212
• Nom complet: thioesterase superfamily member 2
• Masse moléculaire calculée: 140 aa, 15 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC000894
• Symbole du gène: THEM2
• Identification du gène (NCBI): 55856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Them2 (also named Acot13) is a homotetrameric fatty acyl-CoA thioesterase. Them2 is enriched in liver and oxidative tissues, including BAT, and is primarily associated with mitochondria.

{{ptg:RelatedPrimaryAntibodies}}