Anticorps Polyclonal de lapin anti-THOC2

• Phare
• Validé par KD/KO

THOC2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55178-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55178-1-AP (same clone as 55178-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

  IP result of anti-THOC2 (IP:55178-1-AP, 4ug; Detection:55178-1-AP 1:600) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC2 antibody (55178-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

  Immunofluorescent analysis of HeLa cells using 55178-1-AP (THOC2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55178-1-PBS cible THOC2 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: THO complex 2
• Masse moléculaire calculée: 183 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_020449
• Symbole du gène: THOC2
• Identification du gène (NCBI): 57187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.

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