Anticorps Polyclonal de lapin anti-THOC7

• Phare
• Validé par KD/KO

THOC7 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 17881-1-AP

Synonymes

fSAP24, hTREX30, NIF3L1 binding protein 1, NIF3L1BP1, THO complex subunit 7 homolog, THOC7

---

Galerie de données de validation

• 

  WB analysis using 17881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 17881-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17881-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17881-1-AP (THOC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17881-1-AP (THOC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17881-1-AP (THOC7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17881-1-AP (THOC7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17881-1-AP (THOC7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17881-1-AP (THOC7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A431 using 17881-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 17881-1-AP (THOC7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu hépatique humain, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

17881-1-AP cible THOC7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: THOC7 Protéine recombinante Ag11795
• Nom complet: THO complex 7 homolog (Drosophila)
• Masse moléculaire calculée: 204 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC065012
• Symbole du gène: THOC7
• Identification du gène (NCBI): 80145
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

THOC7 is a member of the THO complex that associate with the mRNA export apparatus. It is required for efficient export of polyadenylated RNA. It acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.

Publications

Species	Application	Title
human	WB	Viruses; Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.; Authors - Toshie Sakuma ; KD Validated