Anticorps Monoclonal anti-THRA

• Phare
• Validé par KD/KO

THRA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A11C8

N° de cat : 66703-1-PBS

Synonymes

1A11C8, AR7, c-erbA-1, c-erbA-alpha, EAR 7

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Galerie de données de validation

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  WB analysis of A431 using 66703-1-Ig (same clone as 66703-1-PBS)

  WB result of THRA antibody (66703-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THRA transfected A431 cells. This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66703-1-Ig (same clone as 66703-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using THRA antibody (66703-1-Ig, Clone: 1A11C8 ) at dilution of 1:1300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66703-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66703-1-PBS cible THRA dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: THRA Protéine recombinante Ag27189
• Nom complet: thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
• Masse moléculaire calculée: 490 aa, 54 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC002728
• Symbole du gène: THRA
• Identification du gène (NCBI): 7067
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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