Anticorps Polyclonal de lapin anti-THRAP3

THRAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 19744-1-AP

Synonymes

TRAP150, Thyroid hormone receptor-associated protein complex 150 kDa component, Thyroid hormone receptor-associated protein 3, BCLAF2, BCLAF1 and THRAP3 family member 2

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Galerie de données de validation

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  WB analysis using 19744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19744-1-AP (THRAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19744-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19744-1-AP (THRAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19744-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19744-1-AP (THRAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19744-1-AP

  IP result of anti-THRAP3 (IP:19744-1-AP, 4ug; Detection:19744-1-AP 1:500) with HeLa cells lysate 600ug.

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  IHC staining of human ovary tumor using 19744-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19744-1-AP (THRAP3 Antibody) at dilution of 1:50.

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  IHC staining of human thyroid cancer using 19744-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19744-1-AP (THRAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 19744-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19744-1-AP (THRAP3 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 19744-1-AP

  Immunofluorescent analysis of HepG2 cells using 19744-1-AP (THRAP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules LNCaP
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

19744-1-AP cible THRAP3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: thyroid hormone receptor associated protein 3
• Masse moléculaire calculée: 109 kDa
• Poids moléculaire observé: 109-130 kDa
• Numéro d’acquisition GenBank: NM_005119
• Symbole du gène: THRAP3
• Identification du gène (NCBI): 9967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

THRAP3, also named as TRAP150, plays a role in transcriptional coactivation. The antibody recognizes the C-term of THRAP3.

Publications

Species	Application	Title
pig	WB	Nucleic Acids Res; The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.; Authors - Xin Zong
	IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
human	WB	Cancer Res; NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer; Authors - Ruihui Xie
human	WB,CoIP	Mol Cell Proteomics; Deciphering the clinical significance and kinase functions of GSK3α in colon cancer by proteomics and phosphoproteomics; Authors - Li Gao
human	WB,IF,CoIP	Commun Biol; NLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing; Authors - Zhongliang Chen

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