Anticorps Recombinant de lapin anti-Thyroid Hormone Receptor beta

Thyroid Hormone Receptor beta Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240619E7

N° de cat : 83866-2-PBS

Synonymes

THRB, THR1, NR1A2, ERBA2, c-erbA-beta

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Galerie de données de validation

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  WB analysis of mouse pancreas using 83866-2-RR (same clone as 83866-2-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 83866-2-RR (Thyroid Hormone Receptor beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83866-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83866-2-RR (same clone as 83866-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Thyroid Hormone Receptor beta antibody (83866-2-RR, Clone: 240619E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83866-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83866-2-RR (same clone as 83866-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thyroid Hormone Receptor beta Recombinant Antibody (83866-2-RR, Clone:240619E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83866-2-PBS in a different storage buffer formulation.

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Informations sur le produit

83866-2-PBS cible Thyroid Hormone Receptor beta dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Thyroid Hormone Receptor beta Protéine recombinante Ag16276
• Nom complet: thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian)
• Masse moléculaire calculée: 476 aa, 54 kDa
• Poids moléculaire observé: 53 kDa and 54 kDa
• Numéro d’acquisition GenBank: BC106929
• Symbole du gène: Thyroid Hormone Receptor beta
• Identification du gène (NCBI): 7068
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Thyroid hormone receptor beta (THRB), also known as ERBA2, NR1A2, and THR1, belongs to the nuclear hormone receptor family and NR1 subfamily. THRB is a nuclear hormone receptor that can act as a repressor or activator of transcription. Moreover, THRB is also a high-affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. THRB is the most abundant THR isoform in the liver but is also expressed in other tissues and mediates the effects of THs on hepatic carbohydrate and lipid metabolism (PMID: 34884910). THRB has 2 isoforms with the molecular mass of 53 and 54 kDa (refer to UniProt).

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