Anticorps Polyclonal de lapin anti-THUMPD3

THUMPD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 19807-1-AP

Synonymes

EC:2.1.1.256, THUMP domain containing 3, THUMP domain-containing protein 3, tRNA (guanine(6)-N2)-methyltransferase THUMP3

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Galerie de données de validation

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  WB analysis using 19807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19807-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 19807-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19807-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 19807-1-AP

  Immunofluorescent analysis of Hela cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 19807-1-AP

  Immunofluorescent analysis of HepG2 cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules L02, tissu rénal de souris
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19807-1-AP cible THUMPD3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: THUMPD3 Protéine recombinante Ag13833
• Nom complet: THUMP domain containing 3
• Masse moléculaire calculée: 507 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC001622
• Symbole du gène: THUMPD3
• Identification du gène (NCBI): 25917
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

THUMPD3, or THUMP domain containing 3, is a protein-coding gene that plays a significant role in tRNA methylation, a post-transcriptional modification that affects tRNA biology and is closely associated with human diseases. THUMPD3 is responsible for catalyzing the formation of N2-methylguanosine (m2G) at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs.

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