Anticorps Monoclonal anti-TIA1

TIA1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C11F4

N° de cat : 68486-1-PBS

Synonymes

RNA binding protein TIA 1, p40-TIA-1, p40 TIA 1, Nucleolysin TIA-1 isoform p40, Cytotoxic granule associated RNA binding protein TIA1

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Galerie de données de validation

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  WB analysis using 68486-1-Ig (same clone as 68486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68486-1-Ig (TIA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 68486-1-Ig (same clone as 68486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68486-1-Ig (TIA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68486-1-Ig (same clone as 68486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68486-1-Ig (TIA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68486-1-Ig (same clone as 68486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68486-1-Ig (TIA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 68486-1-Ig (same clone as 68486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68486-1-Ig (TIA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 68486-1-Ig (same clone as 68486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68486-1-Ig (TIA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51061-1

  Cytometric bead array standard curve of MP51061-1, TIA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68486-2-PBS. Detection antibody: 68486-1-PBS. Standard:Ag2778. Range: 0.098-12.5 ng/mL.

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  Cytometric bead array standard curve of MP51061-2

  Cytometric bead array standard curve of MP51061-2, TIA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68486-3-PBS. Detection antibody: 68486-1-PBS. Standard:Ag2778. Range: 0.391-50 ng/mL.

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Informations sur le produit

68486-1-PBS cible TIA1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TIA1 Protéine recombinante Ag2778
• Nom complet: TIA1 cytotoxic granule-associated RNA binding protein
• Masse moléculaire calculée: 214 aa, 24 kDa, 43 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC015944
• Symbole du gène: TIA1
• Identification du gène (NCBI): 7072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.

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