Anticorps Polyclonal de lapin anti-TIAM2

TIAM2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 26870-1-AP

Synonymes

Rho guanine nucleotide exchange factor TIAM2, SIF and TIAM1-like exchange factor, STEF, TIAM 2, TIAM-2

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Galerie de données de validation

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  WB analysis using 26870-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26870-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 26870-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIAM2 antibody (26870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 26870-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human TIAM2 (26870-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HEK-293, cellules U2OS, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules RAW 264.7,
• Résultats positifs en FC (Intra): cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26870-1-AP cible TIAM2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIAM2 Protéine recombinante Ag25469
• Nom complet: T-cell lymphoma invasion and metastasis 2
• Poids moléculaire observé: 190-200 kDa
• Numéro d’acquisition GenBank: BC137275
• Symbole du gène: TIAM2
• Identification du gène (NCBI): 26230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells.

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