Anticorps Polyclonal de lapin anti-TIAM2

TIAM2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26870-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

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  WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 26870-1-AP (same clone as 26870-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIAM2 antibody (26870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 26870-1-AP (same clone as 26870-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human TIAM2 (26870-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26870-1-PBS cible TIAM2 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIAM2 Protéine recombinante Ag25469
• Nom complet: T-cell lymphoma invasion and metastasis 2
• Poids moléculaire observé: 190-200 kDa
• Numéro d’acquisition GenBank: BC137275
• Symbole du gène: TIAM2
• Identification du gène (NCBI): 26230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells.

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