Anticorps Monoclonal anti-TIAR

TIAR Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E8D12

N° de cat : 66907-1-PBS

Synonymes

TIAL1, 1E8D12, Nucleolysin TIAR, TCBP, TIA 1 related protein

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Galerie de données de validation

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  WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66907-1-Ig (same clone as 66907-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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  WB analysis using 66907-1-Ig (same clone as 66907-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66907-1-Ig (same clone as 66907-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66907-1-Ig (TIAR antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 66907-1-Ig (same clone as 66907-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human TIAR (66907-1-Ig, Clone:1E8D12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66907-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66907-1-PBS cible TIAR dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TIAR Protéine recombinante Ag11981
• Nom complet: TIA1 cytotoxic granule-associated RNA binding protein-like 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 39 and 41 kDa
• Numéro d’acquisition GenBank: BC030025
• Symbole du gène: TIAR
• Identification du gène (NCBI): 7073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites. 66907-1-Ig detects 39 and 41 kDa bands in SDS-PAGE. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)

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