Anticorps Polyclonal de lapin anti-TIGAR

• Phare
• Validé par KD/KO

TIGAR Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 22136-1-AP

Synonymes

C12orf5, TP53-induced glycolysis regulatory phosphatase, TP53-induced glycolysis and apoptosis regulator, Fructose-2,6-bisphosphatase TIGAR, EC:3.1.3.46

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Galerie de données de validation

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  WB analysis using 22136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22136-1-AP (TIGAR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu de côlon de souris, tissu d'intestin grêle de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below

Informations sur le produit

22136-1-AP cible TIGAR dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIGAR Protéine recombinante Ag17532
• Nom complet: chromosome 12 open reading frame 5
• Masse moléculaire calculée: 270 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC012340
• Symbole du gène: TIGAR
• Identification du gène (NCBI): 57103
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival.

Publications

Species	Application	Title
mouse	WB	Autophagy; TIGAR exacerbates obesity by triggering LRRK2-mediated defects in macroautophagy and chaperone-mediated autophagy in adipocytes; Authors - Tian Zhang
mouse	WB	Free Radic Biol Med; Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis Pigmentosa.; Authors - Yogita Kanan
mouse	WB	Neurobiol Dis; Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring.; Authors - Amanda R Burmeister
human	WB	Cell Death Dis; MiR-652-5p elevated glycolysis level by targeting TIGAR in T-cell acute lymphoblastic leukemia.; Authors - Shan Liu
mouse	WB	Biochem Biophys Res Commun; Cordycepin exhibits anti-fatigue effect via activating TIGAR/SIRT1/PGC-1α signaling pathway; Authors - Xiaoming Chai
human,mouse	WB	Phytother Res; Dual targeting of wild-type p53 and gut microbiota by Magnolol represses key metabolic process and kills CRC cells; Authors - Haixia Ji

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