Anticorps Recombinant de lapin anti-TIGAR

TIGAR Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242625E7

N° de cat : 85052-4-PBS

Synonymes

C12orf5, 242625E7, EC:3.1.3.46, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator

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Galerie de données de validation

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  WB analysis using 85052-4-RR (same clone as 85052-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85052-4-RR (TIGAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85052-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 85052-4-RR (same clone as 85052-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85052-4-RR (TIGAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85052-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 85052-4-RR (same clone as 85052-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85052-4-RR (TIGAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85052-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85052-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85052-4-RR against Human TIGAR were performed. The affinity constant is 0.308 nM.

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Informations sur le produit

85052-4-PBS cible TIGAR dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TIGAR Protéine recombinante Ag17532
• Nom complet: chromosome 12 open reading frame 5
• Masse moléculaire calculée: 270 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC012340
• Symbole du gène: TIGAR
• Identification du gène (NCBI): 57103
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival.

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