Anticorps Recombinant de lapin anti-TIGIT

TIGIT Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240423D3

N° de cat : 83545-1-PBS

Synonymes

240423D3, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, VSIG9

---

Galerie de données de validation

• 

  WB analysis using 83545-1-RR (same clone as 83545-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83545-1-RR (TIGIT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83545-1-RR (same clone as 83545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83545-1-RR (TIGIT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 83545-1-RR (same clone as 83545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TIGIT antibody (83545-1-RR, Clone: 240423D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 83545-1-RR (same clone as 83545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TIGIT antibody (83545-1-RR, Clone: 240423D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83545-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83545-1-RR against Human TIGIT were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

83545-1-PBS cible TIGIT dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TIGIT Protéine recombinante Eg0749
• Nom complet: T cell immunoreceptor with Ig and ITIM domains
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: NM_173799.4
• Symbole du gène: TIGIT
• Identification du gène (NCBI): 201633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).

{{ptg:RelatedPrimaryAntibodies}}