Anticorps Recombinant de lapin anti-TIGIT

TIGIT Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240423C7

N° de cat : 98023-2-RR

Synonymes

240423C7, T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3

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Galerie de données de validation

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  FC experiment of human PBMCs using 98023-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-2-RR, Clone: 240423C7) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD45RO. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using 98023-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-2-RR, Clone: 240423C7) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.

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  Affinity and Kinetic Characterization of 98023-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98023-2-RR against Human TIGIT were performed. The affinity constant is 5.45 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98023-2-RR cible TIGIT dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: T cell immunoreceptor with Ig and ITIM domains
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: NM_173799.4
• Symbole du gène: TIGIT
• Identification du gène (NCBI): 201633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).

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