Anticorps Recombinant de lapin anti-TIGIT

TIGIT Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240242G1

N° de cat : 98026-1-RR

Synonymes

240242G1, Vstm3

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Galerie de données de validation

• 

  FC experiment of mouse splenocytes using 98026-1-RR

  1x10^6 untreated or CD3/CD28 treated mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug Anti-Mouse TIGIT Rabbit Recombinant Antibody (98026-1-RR, Clone:240242G1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98026-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98026-1-RR against Mouse TIGIT were performed. The affinity constant is 1.08 nM.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 stimulated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98026-1-RR cible TIGIT dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: T cell immunoreceptor with Ig and ITIM domains
• Masse moléculaire calculée: 26kDa
• Numéro d’acquisition GenBank: NP_001139797.1
• Symbole du gène: Tigit
• Identification du gène (NCBI): 100043314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).

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