Anticorps Recombinant de lapin anti-TIGIT

TIGIT Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250731C10

N° de cat : 98026-4-PBS

Synonymes

Vstm3

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98026-4-RR (same clone as 98026-4-PBS)

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse TIGIT Rabbit RecAb (98026-4-RR, Clone: 250731C10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L. Cells were then stained with APC Anti-Human CD4. Cells were not fixed. This data was developed using the same antibody clone with 98026-4-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98026-4-RR (same clone as 98026-4-PBS)

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse TIGIT Rabbit RecAb (98026-4-RR, Clone: 250731C10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse TIGIT Rabbit RecAb (98026-4-RR, Clone: 250731C10) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98026-4-PBS in a different storage buffer formulation.

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Informations sur le produit

98026-4-PBS cible TIGIT dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DNA
• Nom complet: T cell immunoreceptor with Ig and ITIM domains
• Masse moléculaire calculée: 26kDa
• Numéro d’acquisition GenBank: NP_001139797.1
• Symbole du gène: Tigit
• Identification du gène (NCBI): 100043314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).

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