Anticorps Recombinant de lapin anti-TIGIT

TIGIT Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

240423C7

N° de cat : APC-98023-2

Synonymes

240423C7, T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3

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Galerie de données de validation

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  FC experiment of human PBMCs using APC-98023-2

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD45RO (UCHL1) (FITC-65150, Clone: UCHL1), and 5 ul APC Anti-Human TIGIT Rabbit RecAb (APC-98023-2, Clone: 240423C7) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9). Cells were not fixed.

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  FC experiment of human PBMCs using APC-98023-2

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human TIGIT Rabbit RecAb (APC-98023-2, Clone: 240423C7) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-98023-2 cible TIGIT dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TIGIT Protéine recombinante Eg0749
• Nom complet: T cell immunoreceptor with Ig and ITIM domains
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: NM_173799.4
• Symbole du gène: TIGIT
• Identification du gène (NCBI): 201633
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).

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