Anticorps Recombinant de lapin anti-TIM3

• Phare
• Validé par KD/KO

TIM3 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

5A8

N° de cat : 82836-1-RR

Synonymes

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

---

Galerie de données de validation

• 

  WB analysis using 82836-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82836-1-RR (TIM3 antibody) at dilution of 1:3500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 82836-1-RR

  WB result of TIM3 antibody (82836-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIM3 transfected PC-3 cells.

• 

  Affinity and Kinetic Characterization of 82836-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82836-1-RR against Human TIM3 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules PC-3, cellules Daudi, cellules RAW 264.7, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:7000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82836-1-RR cible TIM3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TIM3 Protéine recombinante Ag2459
• Nom complet: hepatitis A virus cellular receptor 2
• Masse moléculaire calculée: 301 aa, 33 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC020843
• Symbole du gène: TIM3
• Identification du gène (NCBI): 84868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

{{ptg:RelatedPrimaryAntibodies}}