Anticorps Polyclonal de lapin anti-TIM3

TIM3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 11872-1-PBS

Synonymes

CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3

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Galerie de données de validation

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  WB analysis using 11872-1-AP (same clone as 11872-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11872-1-AP (TIM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11872-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 11872-1-AP (same clone as 11872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11872-1-AP (TIM3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11872-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 11872-1-AP (same clone as 11872-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TIM3 antibody (11872-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11872-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11872-1-PBS cible TIM3 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIM3 Protéine recombinante Ag2459
• Nom complet: hepatitis A virus cellular receptor 2
• Masse moléculaire calculée: 301 aa, 33 kDa
• Poids moléculaire observé: 50-70 kDa, 33 kDa
• Numéro d’acquisition GenBank: BC020843
• Symbole du gène: TIM3
• Identification du gène (NCBI): 84868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301)

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