Anticorps Monoclonal anti-TIM3

TIM3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

4C4G3

N° de cat : 60355-1-PBS

Synonymes

4C4G3, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3

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Galerie de données de validation

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  WB analysis of Daudi using 60355-1-Ig (same clone as 60355-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

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  WB analysis of pig thymus using 60355-1-Ig (same clone as 60355-1-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 60355-1-Ig (same clone as 60355-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 60355-1-Ig (same clone as 60355-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 60355-1-Ig (same clone as 60355-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 60355-1-Ig (same clone as 60355-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 60355-1-Ig (same clone as 60355-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60355-1-PBS cible TIM3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TIM3 Protéine recombinante Ag16901
• Nom complet: hepatitis A virus cellular receptor 2
• Masse moléculaire calculée: 301 aa, 33 kDa
• Poids moléculaire observé: 33 kDa, 50-70 kDa
• Numéro d’acquisition GenBank: BC020843
• Symbole du gène: TIM3
• Identification du gène (NCBI): 84868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

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