Anticorps Recombinant de lapin anti-TIM3

TIM3 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

241422H1

N° de cat : CL594-98115

Synonymes

241422H1, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3

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Galerie de données de validation

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  FC experiment of human PBMCs using CL594-98115

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with APC Anti-Human CD4 and 5 ul CoraLite®594 Anti-Human TIM3 Rabbit RecAb (CL594-98115, Clone: 241422H1). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL594-98115

  1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human TIM3 Rabbit RecAb (CL594-98115, Clone: 241422H1) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9)(blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human TIM3 Rabbit RecAb (CL594-98115, Clone: 241422H1) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-98115 cible TIM3 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: hepatitis A virus cellular receptor 2
• Masse moléculaire calculée: 301 aa, 33 kDa
• Numéro d’acquisition GenBank: BC020843
• Symbole du gène: TIM3
• Identification du gène (NCBI): 84868
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920).

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