Anticorps Polyclonal de lapin anti-TIM4

TIM4 Polyclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 12008-1-AP

Synonymes

TIMD4, SMUCKLER, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4

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Galerie de données de validation

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  IHC staining of human tonsillitis using 12008-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12008-1-AP (TIM4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12008-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12008-1-AP (TIM4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12008-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12008-1-AP (TIM4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12008-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12008-1-AP (TIM4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 12008-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TIM4 antibody (12008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 12008-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TIM4 antibody (12008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

12008-1-AP cible TIM4 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIM4 Protéine recombinante Ag2621
• Nom complet: T-cell immunoglobulin and mucin domain containing 4
• Masse moléculaire calculée: 378 aa, 42 kDa
• Numéro d’acquisition GenBank: BC008988
• Symbole du gène: TIMD4
• Identification du gène (NCBI): 91937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
goat		Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
mouse	IF	Theranostics; Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages; Authors - Xueqin Li
human	IHC	Front Oncol; Single-Cell RNA-seq Reveals Characteristics of Malignant Cells and Immune Microenvironment in Subcutaneous Panniculitis-Like T-Cell Lymphoma.; Authors - Zifeng Li
mouse	IHC	Biomedicines; Comparative Proteomic Analysis of tPVAT during Ang II Infusion.; Authors - Xiuying Liang
mouse	IF	Liver Int; Regulation of the macrophage-hepatic stellate cell interaction by targeting macrophage PPARγ to prevent NASH progression in mice; Authors - Xi-Xi Ni
human	WB	Int Immunopharmacol; Human leukocyte antigen DR alpha inhibits renal cell carcinoma progression by promoting the polarization of M2 macrophages to M1 via the NF-κB pathway; Authors - Feng Xiong

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