Anticorps Monoclonal anti-TIMELESS

• Phare
• Validé par KD/KO

TIMELESS Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B6G3

N° de cat : 67022-1-PBS

Synonymes

hTIM, Protein timeless homolog, TIM, TIM1, timeless homolog (Drosophila)

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Galerie de données de validation

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  WB analysis using 67022-1-Ig (same clone as 67022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67022-1-Ig (same clone as 67022-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of JAR using 67022-1-Ig (same clone as 67022-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67022-1-Ig (same clone as 67022-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67022-1-Ig (same clone as 67022-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67022-1-Ig (same clone as 67022-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 67022-1-Ig (same clone as 67022-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 67022-1-Ig (same clone as 67022-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human TIMELESS (67022-1-Ig, Clone:1B6G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67022-1-PBS cible TIMELESS dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TIMELESS Protéine recombinante Ag6283
• Nom complet: timeless homolog (Drosophila)
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC050557
• Symbole du gène: TIMELESS
• Identification du gène (NCBI): 8914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

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