Anticorps Polyclonal de lapin anti-TIMM13

TIMM13 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11973-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 11973-1-AP (same clone as 11973-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11973-1-AP (TIMM13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11973-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11973-1-AP (same clone as 11973-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11973-1-AP (TIMM13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11973-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11973-1-AP (same clone as 11973-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11973-1-AP (TIMM13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11973-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11973-1-AP (same clone as 11973-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11973-1-AP (TIMM13 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11973-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11973-1-AP (same clone as 11973-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11973-1-AP (TIMM13 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11973-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11973-1-PBS cible TIMM13 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIMM13 Protéine recombinante Ag2596
• Nom complet: translocase of inner mitochondrial membrane 13 homolog (yeast)
• Masse moléculaire calculée: 95 aa, 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC008607
• Symbole du gène: TIMM13
• Identification du gène (NCBI): 26517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TIMM13 gene, also known as TIM13, TIM13B, TIM M13A or TIMM13B, encodes mitochondrial import inner membrane translocase subunit Tim13 belonging to the small Tim family. TIM13 functions as mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Proteins of the intermembrane space (IMS) of mitochondria are typically synthesized without presequences. TIM13 contains four conserved cysteine residues that bind a zinc ion as cofactor. Import of TIM13 did not depend on the membrane potential or ATP hydrolysis. Upon import into mitochondria TIM13 adopted a stably folded conformation in the IMS.

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