Anticorps Monoclonal anti-TIMM44

• Phare
• Validé par KD/KO

TIMM44 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2c

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4G7D4

N° de cat : 66149-1-PBS

Synonymes

4G7D4, MIMT44, TIM44, TIMM 44

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Galerie de données de validation

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  WB analysis using 66149-1-Ig (same clone as 66149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66149-1-Ig (TIMM44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66149-1-Ig (same clone as 66149-1-PBS)

  WB result of TIMM44 antibody (66149-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIMM44 transfected HeLa cells. This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66149-1-Ig (same clone as 66149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66149-1-Ig (TIMM44 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66149-1-Ig (same clone as 66149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66149-1-Ig (TIMM44 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66149-1-Ig (same clone as 66149-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIMM44 antibody (66149-1-Ig, Clone: 4G7D4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66149-1-PBS cible TIMM44 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2c
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TIMM44 Protéine recombinante Ag21389
• Nom complet: translocase of inner mitochondrial membrane 44 homolog (yeast)
• Masse moléculaire calculée: 452 aa, 51 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC033628
• Symbole du gène: TIMM44
• Identification du gène (NCBI): 10469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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