Anticorps Recombinant de lapin anti-TIMP1

TIMP1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

243010H8

N° de cat : 85959-1-RR

Synonymes

Clgi, Timp, TIMP 1

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Galerie de données de validation

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  WB analysis using 85959-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85959-1-RR (Timp1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 85959-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85959-1-RR (TIMP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 85959-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85959-1-RR (TIMP1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 85959-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TIMP1 antibody (85959-1-RR, Clone: 243010H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85959-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85959-1-RR against Mouse TIMP1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules C2C12, tissu ovarien de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85959-1-RR cible TIMP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TIMP1 Protéine recombinante Eg0630
• Nom complet: tissue inhibitor of metalloproteinase 1
• Masse moléculaire calculée: 23KD
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: NM_001044384.1
• Symbole du gène: Timp1
• Identification du gène (NCBI): 21857
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.

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