Anticorps Recombinant de lapin anti-TIMP1

TIMP1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230329E7

N° de cat : 83151-5-PBS

Synonymes

TIMP-1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, Collagenase inhibitor

---

Galerie de données de validation

• 

  IF Staining of HeLa using 83151-5-RR (same clone as 83151-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83151-5-RR (same clone as 83151-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HT-29 using 83151-5-RR (same clone as 83151-5-PBS)

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

• 

  FC experiment of MDA-MB-231 using 83151-5-RR (same clone as 83151-5-PBS)

  1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83151-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83151-5-RR against Human TIMP1 were performed. The affinity constant is 1.33 nM.

---

Informations sur le produit

83151-5-PBS cible TIMP1 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: TIMP metallopeptidase inhibitor 1
• Masse moléculaire calculée: 23 kDa
• Numéro d’acquisition GenBank: BC000866
• Symbole du gène: TIMP1
• Identification du gène (NCBI): 7076
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}