Anticorps Polyclonal de lapin anti-TIMP-4

TIMP-4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 12326-1-AP

Synonymes

TIMP4, TIMP 4, Metalloproteinase inhibitor 4

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Galerie de données de validation

• 

  WB analysis of fetal human brain using 12326-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12326-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12326-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12326-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12326-1-AP (TIMP-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12326-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 12326-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12326-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12326-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12326-1-AP (TIMP-4 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 12326-1-AP

  Immunofluorescent analysis of MCF-7 cells using 12326-1-AP (TIMP-4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules BxPC-3, cellules HT-1080, tissu cardiaque humain
• Résultats positifs en IHC: tissu de gliome humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 1 publications below

Informations sur le produit

12326-1-AP cible TIMP-4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIMP-4 Protéine recombinante Ag2927
• Nom complet: TIMP metallopeptidase inhibitor 4
• Masse moléculaire calculée: 224 aa, 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC010553
• Symbole du gène: TIMP4
• Identification du gène (NCBI): 7079
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
mouse	WB,IF	Viruses; Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model.; Authors - Wangquan Ji
human	WB	J Biochem Mol Toxicol; Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling; Authors - Wei Xu
human	WB	Biochem Biophys Res Commun; Molecularly targeted anti-cancer drugs inhibit the invasion and metastasis of hepatocellular carcinoma by regulating the expression of MMP and TIMP gene families.; Authors - Xiao-Xiao He
mouse	WB	PLoS One; Fructose-1,6-bisphosphate prevents pulmonary fibrosis by regulating extracellular matrix deposition and inducing phenotype reversal of lung myofibroblasts.; Authors - Henrique Bregolin Dias
human	WB	Biomed Res Int; Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway.; Authors - Xiaoxiao He

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