Anticorps Polyclonal de lapin anti-TINAG-Specific

TINAG-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 19747-1-AP

Synonymes

TIN AG, TINAG

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Galerie de données de validation

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  WB analysis of mouse small intestine using 19747-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 19747-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 19747-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 19747-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 19747-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 19747-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris, tissu placentaire humain, tissu rénal humain
• Résultats positifs en IHC: tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

19747-1-AP cible TINAG-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tubulointerstitial nephritis antigen
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: NM_014464
• Symbole du gène: TINAG
• Identification du gène (NCBI): 27283
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TINAG belongs to the peptidase C1 family. TINAG mediates adhesion of proximal tubule epithelial cells via integrins alpha3-beta1 and alphaV-beta3. It is a non catalytic peptidase C1 family protein. Antibodies against TINAG are found in sera of patients with tubulointerstitial nephritis, a rare autoimmune disorder that causes acute and chronic renal injury.It has 2 isoforms produced by alternative splicing. The antibody is specific to TINAG.

Publications

Species	Application	Title
human	IHC	Transl Cancer Res; Modeling of new markers for the diagnosis and prognosis of pancreatic cancer based on the transition from inflammation to cancer; Authors - Yuan Zhou

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