Anticorps Monoclonal anti-TINP1

TINP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E3B11

N° de cat : 66374-1-PBS

Synonymes

HUSSY-29, HUSSY29, HCL G1, Hairy cell leukemia protein 1, Hairy cell leukemia

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Galerie de données de validation

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  WB analysis of L02 using 66374-1-Ig (same clone as 66374-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66374-1-Ig (same clone as 66374-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66374-1-PBS cible TINP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TINP1 Protéine recombinante Ag8822
• Nom complet: TGF beta-inducible nuclear protein 1
• Masse moléculaire calculée: 260 aa, 30 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC005288
• Symbole du gène: TINP1
• Identification du gène (NCBI): 10412
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.

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