Anticorps Monoclonal anti-TLE3

TLE3 Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D11A8

N° de cat : 66083-1-PBS

Synonymes

1D11A8, Enhancer of split groucho-like protein 3, ESG, ESG3, GRG 3

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 66083-1-Ig (same clone as 66083-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66083-1-Ig (same clone as 66083-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 66083-1-Ig (same clone as 66083-1-PBS)

  IP result of anti-TLE3 (IP:66083-1-Ig, 5ug; Detection:66083-1-Ig 1:500) with HEK-293 cells lysate 1480ug. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66083-1-Ig (same clone as 66083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66083-1-Ig (same clone as 66083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66083-1-PBS cible TLE3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TLE3 Protéine recombinante Ag17906
• Nom complet: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Masse moléculaire calculée: 772 aa, 83 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC015729
• Symbole du gène: TLE3
• Identification du gène (NCBI): 7090
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )

{{ptg:RelatedPrimaryAntibodies}}