Anticorps Monoclonal anti-TLR2

• Phare
• Validé par KD/KO

TLR2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C1D3

N° de cat : 66645-1-PBS

Synonymes

CD282, TIL4, toll like receptor 2, Toll/interleukin-1 receptor-like protein 4, Toll-like receptor 2

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Galerie de données de validation

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  WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

  WB result of TLR2 antibody (66645-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR2 transfected THP-1 cells. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 66645-1-Ig (same clone as 66645-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66645-1-Ig (same clone as 66645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66645-1-Ig (TLR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR2 antibody (66645-1-Ig, Clone: 1C1D3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50388-1

  Cytometric bead array standard curve of MP50388-1, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-2-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50388-3

  Cytometric bead array standard curve of MP50388-3, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-4-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

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Informations sur le produit

66645-1-PBS cible TLR2 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TLR2 Protéine recombinante Ag25626
• Nom complet: toll-like receptor 2
• Masse moléculaire calculée: 784 aa, 90 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC033756
• Symbole du gène: TLR2
• Identification du gène (NCBI): 7097
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).

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