Anticorps Recombinant de lapin anti-TLR3/CD283

TLR3/CD283 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230445G4

N° de cat : 83136-3-PBS

Synonymes

TLR3, 230445G4, CD283, toll like receptor 3, Toll-like receptor 3

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Galerie de données de validation

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  WB analysis using 83136-3-RR (same clone as 83136-3-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83136-3-RR (same clone as 83136-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83136-3-RR (same clone as 83136-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83136-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83136-3-PBS cible TLR3/CD283 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TLR3/CD283 Protéine recombinante Ag34044
• Nom complet: toll-like receptor 3
• Masse moléculaire calculée: 904 aa, 104 kDa
• Poids moléculaire observé: 120 kDa, 68 kDa
• Numéro d’acquisition GenBank: BC094737
• Symbole du gène: TLR3
• Identification du gène (NCBI): 7098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679). Western detected full-length (∼120 kD) and cleaved (∼70 kDa) TLR3 (PMID: 28717003).

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