Anticorps Recombinant de lapin anti-TLR3/CD283

TLR3/CD283 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

230445G4

Avis (1)

N° de cat : 83136-3-RR

Synonymes

TLR3, 230445G4, CD283, toll like receptor 3, Toll-like receptor 3

---

Galerie de données de validation

• 

  WB analysis using 83136-3-RR

  HT-29 cells were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovarian cancer using 83136-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovarian cancer using 83136-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 83136-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 83136-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83136-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules HT-29, tissu placentaire humain
• Résultats positifs en IHC: human ovarian cancer, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

83136-3-RR cible TLR3/CD283 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TLR3/CD283 Protéine recombinante Ag34044
• Nom complet: toll-like receptor 3
• Masse moléculaire calculée: 904 aa, 104 kDa
• Poids moléculaire observé: 120 kDa, 68 kDa
• Numéro d’acquisition GenBank: BC094737
• Symbole du gène: TLR3
• Identification du gène (NCBI): 7098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679). Western detected full-length (∼120 kD) and cleaved (∼70 kDa) TLR3 (PMID: 28717003).

Publications

Species	Application	Title
human	WB	NAR Mol Med; C G composition in transposon-derived genes is increased in FXD with perturbed immune system; Authors - Tamaki Suganuma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Iustina (Verified Customer) (04-24-2025)

I have tried other Antibodies for TLR3 detection, but from all of them only this product detected the right size band: 120 kDa. Other products detected protein at 67-72 kDa, whereas with this product, I managed two bands: one at 120 and one at 67-72 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: BEAS-2B

{{ptg:RelatedPrimaryAntibodies}}