Anticorps Recombinant de lapin anti-TLR4/CD284

TLR4/CD284 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, FC

Conjugaison

Non conjugué

CloneNo.

230463C7

N° de cat : 98018-1-RR

Synonymes

Tlr4, CD284, toll like receptor 4, 230463C7

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Galerie de données de validation

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  FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.

• 

  FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: macrophages péritonéaux de souris Balb/c,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98018-1-RR cible TLR4/CD284 dans les applications de WB, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TLR4/CD284 Protéine recombinante Ag32998
• Nom complet: toll-like receptor 4
• Masse moléculaire calculée: 96 kDa
• Numéro d’acquisition GenBank: NM_021297
• Symbole du gène: Tlr4
• Identification du gène (NCBI): 21898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

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