Anticorps Recombinant de lapin anti-TLR6

TLR6 Recombinant Antibody for IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241926F2

N° de cat : 84811-2-PBS

Synonymes

Toll-like receptor 6, toll like receptor 6, CD286, 241926F2

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP01727-1

  Sandwich ELISA standard curve of MP01727-1, Human TLR6 Recombinant Matched Antibody Pair - PBS only. 84811-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY02306. 84811-2-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

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  Sample test of MP01727-1

  The mean TLR6 concentration was determined to be 166.5 pg/mL in Daudi cell extract based on a 1.8 mg/mL extract load.

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  IF Staining of mouse spleen using 84811-2-RR (same clone as 84811-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TLR6 antibody (84811-2-RR, Clone: 241926F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84811-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 84811-2-RR (same clone as 84811-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TLR6 antibody (84811-2-RR, Clone: 241926F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84811-2-PBS in a different storage buffer formulation.

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Informations sur le produit

84811-2-PBS cible TLR6 dans les applications de IF-P, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: toll-like receptor 6
• Masse moléculaire calculée: 480 aa, 56 kDa
• Numéro d’acquisition GenBank: BC111755
• Symbole du gène: TLR6
• Identification du gène (NCBI): 10333
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TLR6, also known as CD286, belongs to the Toll-like receptor (TLR) family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR6 can form a heterodimer with TLR2. TLR6 and TLR2 are both recruited to the macrophage phagosome, where they recognize peptidoglycan, a Gram-positive pathogen component (PMID: 11095740). TLR6 interacts with CD36, following CD36 stimulation by oxLDL or amyloid-beta 42, and forms a heterodimer with TLR4. The trimeric complex is internalized and triggers an inflammatory response.

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