Anticorps Polyclonal de lapin anti-TLR7

• Phare
• Validé par KD/KO

TLR7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 17232-1-AP

Synonymes

UNQ248/PRO285, Toll-like receptor 7, toll like receptor 7

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Galerie de données de validation

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  WB analysis of mouse lung using 17232-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17232-1-AP (TLR7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17232-1-AP

  WB result of TLR7 antibody (17232-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR7 transfected Jurkat cells.

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  IHC staining of human lung using 17232-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17232-1-AP (TLR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 17232-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17232-1-AP (TLR7 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of RAW 264.7 using 17232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TLR7 antibody (17232-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules Jurkat
• Résultats positifs en IHC: tissu pulmonaire humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

17232-1-AP cible TLR7 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TLR7 Protéine recombinante Ag11073
• Nom complet: toll-like receptor 7
• Masse moléculaire calculée: 1049 aa, 121 kDa
• Poids moléculaire observé: 121 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC033651
• Symbole du gène: TLR7
• Identification du gène (NCBI): 51284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Among the TLR family, TLR7 (toll-like receptor 7) is an endosomal innate immune sensor that can detect single-stranded ribonucleic acid. Constitutive expression of TLR7 is pre-dominant in dendritic cells and B cells compared to other immune cells. Low levels of TLR7 expression have been observed in non-immune cells, such as hepatocytes, epithelial cells, and keratinocytes (PMID: 37596656).

Publications

Species	Application	Title
human	IHC	Nat Commun; TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase; Authors - Gang Liu
rat	WB	Acta Physiol (Oxf); Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.; Authors - Li Zhang
human	WB	Stem Cell Res Ther; Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.; Authors - Ruijie Zhang ; KD Validated
mouse	WB,IF	Mol Neurobiol; Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment; Authors - Liyun Deng
mouse	WB	Front Immunol; Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.; Authors - Lujie Yang
rat	WB	Mol Cell Endocrinol; Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.; Authors - Dongmin Li

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